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| Muzaffar,Adnan; Kiani,Sarfraz; Khan,Muhammad Azmat Ullah; Rao,Abdul Qayyum; Ali,Arfan; Awan,Mudassar Fareed; Iqbal,Adnan; Nasir,Idrees Ahmad; Shahid,Ahmad Ali; Husnain,Tayyab. |
| BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating the target protein at the point of insect attack will be more effective. It will not mean that the non-green parts of the plants are free of toxic proteins, but it will inflict more damage on the insects because they are at maximum activity in the green parts of plants. RESULTS: Successful cloning was achieved by the amplification of Cry2A, Cry1Ac, and a transit peptide. The appropriate polymerase chain reaction amplification and digested products confirmed... |
| Tipo: Journal article |
Palavras-chave: Chloroplast transient peptide; Cry1Ac; Cry2A; Bt; CTP; Cry genes; Endotoxins; GUS; Transgenic plants. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100014 |
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| Kiani,Sarfraz; Ali,Arfan; Bajwa,Kamran Shehzad; Muzaffar,Adnan; Ashraf,Muhammad Aleem; Samiullah,Tahir Rehman; Shahid,Ahmad Ali; Husnain,Tayyab. |
| Background: Transgenic plants inhabiting single Bt gene are prone to develop insect resistance and this resistance has been reported in case of some important yield-devastating insect larvae of commercial crops, such as cotton and rice. Therefore, it has become essential to adapt new strategies to overcome the problem of insect resistance and these new strategies should be sophisticated enough to target such resistant larvae in broad spectrum. Among these, plants may be transformed with Bt gene tagged with some fusion-protein gene that possesses lectin-binding capability to boost the binding sites for crystal protein gene within insect mid-gut in order to overcome any chances of insect tolerance against Bt toxin. Enhanced chloroplast-targeted Bt gene... |
| Tipo: Journal article |
Palavras-chave: Chloroplast expression; Chloroplast transit peptide; Cotton transformation; Confocal microscopy; Ricin b-chain. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600013 |
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| Toufiq,Nida; Tabassum,Bushra; Bhatti,Muhammad Umar; Khan,Anwar; Tariq,Muhammad; Shahid,Naila; Nasir,Idrees Ahmad; Husnain,Tayyab. |
| Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I gene was isolated from the genomic DNA of Barley (Hordeum vulgare L.) cultivar, Haider-93. The isolated DNA was used as template for the amplification of the ∼935 bp full-length chitinase I gene. Based on the sequence of the amplified gene fragment, class I barley chitinase shares 93% amino acid sequence homology with class II wheat chitinase. Interestingly, barley class I chitinase and class II... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antifungal activity assay; Barley chitinase class I; Escherichia coli expression; Recombinant chitinase protein. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200414 |
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| Rahman,Mahmood-ur; Rashid,Hamza; Shahid,Ahmad Ali; Bashir,Khurram; Husnain,Tayyab; Riazuddin,Sheikh. |
| Advanced generations of different transgenic lines of indica basmati rice (Basmati-370) expressing two unrelated Bt genes, cry1Ac and cry2A were evaluated for resistance to Yellow Stem Borer (YSB) and Rice Leaf Folder (RLF) under field conditions compared to control lines over three years (2003-2005). Homozygous lines were selected and analyzed for insect resistance, morphological, physiochemical properties and risk assessment studies. After artificial infestation of target insects, the transgenic plants showed significant resistance. Data were recorded in terms of dead hearts and white heads at vegetative and flowering stage respectively. Transgenic lines showed up to 100 and 96% resistance against yellow stem borer at vegetative and flowering stages,... |
| Tipo: Journal article |
Palavras-chave: Bio-safety; Cry1Ac; Cry2A; Gene flow; Field trials; Gene pyramiding; Insect resistance. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200008 |
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